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Nature 424 (6948): 5615

LC-MS/MS experiment was performed on a 1290 Infinity U-HPLC system (Agilent Technologies, Santa Clara, CA, USA) mounted with a Kinetex Biphenyl column (2.1 mm, 50 mm, 1.7 m, Phenomenex), maintained at 50 C

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Nivolumab was accordingly administered at a dose of 3 mg/kg every 3 weeks in combination with ipilimumab 1 mg/kg every 3 weeks for up to 4 doses, followed by maintenance with single-agent nivolumab 480 mg flat dose every 4 weeks until disease progression or intolerable toxicity